Hybridization
Antigen and Antibody Reaction
Reaction solution
Extract Solution
Blood,
Mouth cell
BISTnnerTM(BIST)
Multiplex Signal Detection system
for ELISA and DNA typing
using BIST Technology
Technology
Next
Arranged 3 POF
3 directions
Specificity
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Capillary length : 50mm |
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Beads Sizes : ø 1mm |
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Total 43 beads in BIST (18 beads for capture and signal detection + 24 beads for quencher as spacer + 1 bead for negative control) |
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Reaction Volume : approx.40μl |
BIST Detection system － BISTnner －
SNP typing Antigen
Chemiluminescence
Top View
BIST can incorporate multiplex reaction analysis both manually and fully
automated of various biomolecules
Introduction
BISTTM:Bead array In Single Tip
Data
.Chemiluminescence scan
Beads position by LED silhouette scan
The development of a novel system to provide simple system
and rapid multiplex detection is presented. The system named BIST(Bead
array In Single Tip). BIST incorporates beads (maximum 50pcs, 1mm)
aligned in a capillary tip offering ease of use and the option to
perform the system manually
or automated. There are two kinds of beads incorporated into BIST.
One for capture and signal detection
with the other functioning as a quencher. This feature of BIST allows for
luminescent detection on all non
quencher beads with results incorporating both the beads position and intensity, captured by a unique
signal detection reader called the BISTnner.
We show here that novel BIST 1 system offers unique capabilities
for multiplex analysis in Diagnostic applications.
BIST consists of a capillary tip packed with 30-50 beads(φ1mm）
and can function as a tip for liquid
dispensing. Binding each different biomolecules on the different beads
BIST Applications Process
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s a system for Multiplex Gene analysis using a capillary bead array |
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is a technology that can be fully automated using Magtration Technology |
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is a process to apply gene analysis from pre-sample preparation to detection |
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detects the luminescence intensity on each bead and the beads’ positions |
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offers wide applications for gene and protein analysis |
Reaction to antigen and Washing
Blood, Food,Bacterial cell lysis etc.
Antigen binding Beads
PCR-ASPE
(SNPs detection reaction)
DNA probe binding Beads
Hybridization
Chemiluminescence
Plastic Optical Fiber (POF)
What is BIST
Feature of BIST System
Multiplex assay