In this example, amplicon derived from human genomic DNA containing either SNP site A/A (homozygous A), A/G (heterozygous) or G/G (homozygous G) was prepared and spotted onto a Bio-Strand tip. Each sample was spotted eight times in a row and oligonucleotides corresponding to the SNP site containing either a red (T) or green (C) fluorophore were mixed and hybridized to the tip.

 

False color images in Figures I - III show the result of this experiment. If the SNP site is A, the red fluorophore is detected (Fig.I); if it is G, the green fluorophore signal is obtained (Fig.II). Figure III is a composite of Figures I & II.

The red signal indicates homozygous A, green indicates homozygous G and the yellow signal represents the heterozygote.

 

In this manner, the Bio-Strand system can be used to detect SNPs in genes. Antibodies can also be fixed on the chip, making it useful for proteomic

SNP Analysis (Format I)
SNP Analysis (Format II)