In this example, amplicon derived from human genomic DNA containing either SNP site A/A (homozygous A), A/G (heterozygous) or G/G (homozygous G) was prepared and spotted onto a Bio-Strand tip. Each sample was spotted eight times in a row and oligonucleotides corresponding to the SNP site containing either a red (T) or green (C) fluorophore were mixed and hybridized to the tip.
False color images in Figures I - III show the result of this experiment. If the SNP site is A, the red fluorophore is detected (Fig.I); if it is G, the green fluorophore signal is obtained (Fig.II). Figure III is a composite of Figures I & II.
The red signal indicates homozygous A, green indicates homozygous G and the yellow signal represents the heterozygote.