Precision System Science
8G Compact Liquid Handling Robot
96-plex mitochondria DNA samples
All 11 samples were processed in
(1) lane of Illumina Hi-Seq
~28.5 million reads
Average of 2.6 mil / sample
90% pass filter
|
Sample #
|
Sample
|
Reads
|
|
1
|
05L-3793:
|
3,221,634
|
|
2
|
04L-0808:
|
2,791,873
|
|
3
|
00-3448:
|
1,408,669
|
|
4
|
01-2319:
|
2,866,884
|
|
5
|
99-2079:
|
1,618,123
|
|
6
|
99-7650:
|
2,477,994
|
|
7
|
01-4049:
|
2,852,414
|
|
8
|
97-3605:
|
3,320,296
|
|
9
|
98-5888:
|
1,926,697
|
|
10
|
10-924:
|
3,133,641
|
|
11
|
99-8254:
|
2,969,809
|
|
Total:
|
|
28,588,034
|
Tuberclosis Samples Sequencing Data
The 8GC liquid handling robot can pipette liquids up to 200ul at a time.
(A) It has capability if In-tip magnetic bead separation. Mixing 1 to 8
samples simultaneously, the robot can mix beads into solution. To capture
beads, a magnet is deployed and beads get captured in the tip.
(B) Supernatant then gets pipetted out captued beads can now be washed
and eluted.
Post Amplification chosen at random from 24 preparations
Starting material as low as 30ng
All samples Amplified
~114 million reads of all 96 samples in (1) lane of Illumina
64.4% of reads mapped
All 96 samples had an average of 1665x coverage